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Leonardo Ramón Leggieri
Julieta Soledad de Anna
Gerardo Abel Cerón
Luis Arias Darraz
Daniela Fuentes Monasterio
Juan Guillermo Cárcamo
Carlos Marcelo Luquet


Cytochrome P450 (CYP) gene expressions were studied by semi-quantitative polymerase chain reaction (PCR) to assess oil pollution using liver and gills of juvenile rainbow trout Oncorhynchus mykiss sampled from La Mina stream (Bariloche, Argentina), up and downstream of a discharge of petroleum spill from an unproductive wellbore. Data was complemented with laboratory exposure to water accommodated fraction (WAF) from the oil spill. Oil exposure induced gill and liver CYP1A transcription (1.2-1.4-fold), which was used as reference biomarker. In both organs, CYP2K1 transcript was induced after 5 % WAF exposure, 24 h (1.8-2.0-fold), and it was down-regulated in more extended time (1.2-1.5-fold). CYP2M1 transcript levels were down-regulated in liver of impacted wild trout and in trout exposed to 5 % WAF during 96 h (1.5-1.7-fold). CYP2K1 and CYP2M1 are involved in the metabolism of fatty acids and estrogenic hormones, and its down-regulation would indicate metabolic alterations. CYP3A27 levels were only induced in liver of wild trout (1.6-fold), possibly related to crosstalk between aryl hydrocarbon receptor and pregnane X receptor signaling pathway. While CYP1A mRNA expression appears to be a more robust biomarker for detecting the effects of crude oil under different dose and exposure time, the expression of CYP2K1, CYP2M1 and CYP3A27 mRNAs would indicate different time exposure to oil pollution, particularly under high oil concentration in water. Hence, patterns of multiple CYP genes expression in rainbow trout gills and liver could be especially valuable for monitoring programs of crude oil pollution and remediation actions on Patagonian freshwater ecosystems.


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Leonardo Ramón Leggieri, INIBIOMA - CONICET

Leggieri LR es Licenciado en Ciencias Biológicas y Doctor en Biología de la Universidad Nacional del Comahue (Argentina). Actualmente, desarrolla un posdoctorado en biología molecular solventado por el CONICET y la Fundación YPF en el Instituto de Investigaciones en Biodiversidad y Medioambiente (Bariloche, Argentina).

Julieta Soledad de Anna, Laboratorio de Ecotoxicología Acuática, Instituto de Investigaciones en Biodiversidad y Medioambiente (INIBIOMA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Dirección: Ruta 61, km 3, CCP7, Junín de los Andes (CP8371), Neuquén, Argentina

Licenciada en Ciencias Biológicas con orientación en Biología Molecular y Celular - Realizando un Doctorado en Biología (Universidad Nacional del Comahue, Argentina)

Gerardo Abel Cerón, Laboratorio de Zoología, Centro Regional Universitario Bariloche de la Universidad Nacional del Comahue – Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Dirección: Quintral 1250, San Carlos de Bariloche (CP8400), Río Negro, Argentina

Licenciado en Ciencias Biológicas y Doctor en Biología - Realizando un Posdoctorado (CONICET) 

Luis Arias Darraz, Instituto de Bioquímica y Microbiología, Facultad de Ciencias, Universidad Austral de Chile - Dirección: Campus Isla Teja, Casilla 567, Valdivia, Chile

Bioquímico - Realizando un Doctorado (Universidad Austral de Chile)

Daniela Fuentes Monasterio, Instituto de Bioquímica y Microbiología, Facultad de Ciencias, Universidad Austral de Chile - Dirección: Campus Isla Teja, Casilla 567, Valdivia, Chile


Juan Guillermo Cárcamo, Fondo de Financiamiento de Centros de Investigación en Áreas Prioritarias (FONDAP), Interdisciplinary Center for Aquaculture Research (INCAR) - Instituto de Bioquímica y Microbiología, Facultad de Ciencias, Universidad Austral de Chile - Dirección: Campus Isla Teja, Casilla 567, Valdivia, Chile

Bioquímico, Research Fellow (Memorial Sloan-Kettering Cancer Center, New York USA), Doctor en Ciencias (Universidad Austral de Chile. Post Doctorado (FONDECYT). Categoría: Invetigador Principal (CONICYT)

Carlos Marcelo Luquet, Laboratorio de Ecotoxicología Acuática, Instituto de Investigaciones en Biodiversidad y Medioambiente (INIBIOMA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Dirección: Ruta 61, km 3, CCP7, Junín de los Andes (CP 8371), Neuquén, Argentina

Licenciado en Ciencias Biológicas y Doctor en Ciencias Biológicas (CONICET). Categoría: Investigador Principal (CONICET)

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