A simple tool to assess the effect of water-soluble soil pollutants on enzyme activity in human whole blood samples using WST-1 assay Short Communication

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Vanya Koleva
Teodora Koynova
Ivaila Kuleva
Asya Dragoeva


Hazardous compounds accumulated in soil affect soil quality. Here we show that WST-1 can be used for evaluation of the effect of water soil extracts on enzyme activity in human whole-blood samples. Soil samples from the Shumen Plateau Nature Park (NP) and the City Park in Shumen (SCP) were studied. We observed significant differences between control and treated whole-blood samples. These results indicated that soil water extracts affected normal metabolic activity. In conclusion, the WST-1 assay can be used for biomonitoring soil quality. The presence of harmful water-soluble compounds in tested soils may pose a risk to ecosystems and human health.


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