BIOSORPTION OF CADMIUM AND LEAD USING SUSPENDED AND IMMOBILIZED <em>Enterobacter cloacae</em> AT DIFFERENT pH

Contenido principal del artículo

Carolina Bojórquez
Martín Gabriel Frías-Espericueta
Bruno Gómez-Gil
Domenico Voltolina

Resumen

The aim of this work was to determine the best conditions for biomass production of the bacterium Enterobacter cloacae and for Cd and Pb retention by adsorption on the cell walls or by absorption and accumulation in the bacterial biomass. Suspended and immobilized cultures of this bacterium, acclimated to both metals, were grown for 24 h at pH 6, 7 and 9, in trypticase soy (TS) medium added with 2.73 mg/L of Cd or 16.8 mg/L of Pb. Immobilized cultures at pH 7 and 9 had the highest biomass production. Percentages of Cd retention by adsorption and absorption increased with increasing pH, but adsorption was higher in suspended while absorption was higher in immobilized cultures. Possibly because of different affinity of bacterial cells for these metals, the percentages of Pb retention were higher than those of Cd. In percentage, the highest Pb adsorption on cell walls was at pH 9, while retention by biomass decreased with increasing pH. The amount of metal retained by one g of bacterial biomass, efficiency was higher at pH 6, possibly because of the presence of dead or dying bacterial cells.

 

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Detalles del artículo

Biografía del autor/a

Carolina Bojórquez, Posgrado en Ciencias en Recursos Acuáticos, Facultad de Ciencias del Mar, Universidad Autónoma de Sinaloa, Av. de los Deportes s/n, Ciudad Universitaria, Mazatlán, Sinaloa, C.P. 82000, México

Estudiante de Doctorado

Martín Gabriel Frías-Espericueta, Facultad de Ciencias del Mar, Universidad Autónoma de Sinaloa, Paseo Claussen s/n, Col. Los Pinos, Mazatlán, Sinaloa, C.P. 82000, México

Prof. Inv. Tit. C

Bruno Gómez-Gil, Centro de Investigación en Alimentacion y Desarrollo, Av. Sábalo-Cerritos s/n, Estero del Yugo, Mazatlán, Sinaloa, C.P. 82000, México

Inv. Tit. C

Domenico Voltolina, Centro de Investigaciones Biológicas del Noroeste, Laboratorio UAS-CIBNOR, FAcultad de Ciencias del Mar, Universidad Autónoma de Sinaloa, Paseo Claussen s/n, Col. Los Pinos, Mazatlán, Sinaloa, C.P. 82000, México

inv tit c

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